Specificity and Structural Requirements of Phospholipase C-β Stimulation by Rho GTPases Versus G Protein βγ Dimers *
Journal of Biological Chemistry, ISSN: 0021-9258, Vol: 278, Issue: 5, Page: 3006-3014
2003
- 67Citations
- 41Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations67
- Citation Indexes67
- 67
- CrossRef60
- Captures41
- Readers41
- 41
Article Description
Phospholipase C-β 2 (PLCβ 2 ) is activated both by heterotrimeric G protein α- and βγ- subunits and by Rho GTPases. In this study, activated Rho GTPases are shown to stimulate PLCβ isozymes with the rank order of PLCβ 2 > PLCβ 3 ≥ PLCβ 1. The sensitivity of PLCβ isozymes to Rho GTPases was clearly different from that observed for G protein βγ dimers, which decreased in the following order: PLCβ 3 > PLCβ 2 > PLCβ 1 for β 1 γ 1/2 and PLCβ 2 > PLCβ 1 >>> PLCβ 3 for β 5 γ 2. Rac1 and Rac2 were found to be more potent and efficacious activators of PLCβ 2 than was Cdc42Hs. The stimulation of PLCβ 2 by Rho GTPases and G protein βγ dimers was additive, suggesting that PLCβ 2 activation can be augmented by independent regulation of the enzyme by the two stimuli. Using chimeric PLCβ 1 -PLCβ 2 enzymes, βγ dimers, and Rho GTPases are shown to require different regions of PLCβ 2 to mediate efficient stimulation of the enzyme. Although the catalytic subdomains X and Y of PLCβ 2 were sufficient for efficient stimulation by βγ, the presence of the putative pleckstrin homology domain of PLCβ 2 was absolutely required for the stimulation of the enzyme by Rho GTPases. Taken together, these results identify Rho GTPases as novel PLCβ regulators, which mediate PLCβ isozyme-specific stimulation and are potentially involved in coordinating the activation of PLCβ 2 by extracellular mediators in intact cells.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0021925819308907; http://dx.doi.org/10.1074/jbc.m208282200; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0037474209&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/12441352; https://linkinghub.elsevier.com/retrieve/pii/S0021925819308907; http://www.jbc.org/lookup/doi/10.1074/jbc.M208282200; https://syndication.highwire.org/content/doi/10.1074/jbc.M208282200; https://dx.doi.org/10.1074/jbc.m208282200
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know