Cross-talk between Native Plasmalemmal Na + /Ca 2+ Exchanger and Inositol 1,4,5-Trisphosphate-sensitive Ca 2+ Internal Store in Xenopus Oocytes *
Journal of Biological Chemistry, ISSN: 0021-9258, Vol: 279, Issue: 50, Page: 52414-52424
2004
- 20Citations
- 15Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations20
- Citation Indexes20
- 20
- CrossRef19
- Captures15
- Readers15
- 15
Article Description
Because the presence of a native plasmalemmal Na + /Ca 2+ exchange (NCX) activity in Xenopus laevis oocytes remains controversial, its possible functional role in these cells is poorly understood. Here, in experiments on control oocytes and oocytes overexpressing a cloned NCX1 cardiac protein, confocal microscopy combined with electrophysiological techniques reveal that these cells express an endogenous NCX protein forming a functional microdomain with inositol 1,4,5-trisphosphate receptors (InsP 3 R) that controls intracellular Ca 2+ in a restricted subplasmalemmal space. The following data obtained in control denuded oocytes are consistent with this view: (i) reverse transcription-PCR revealed that the oocyte expresses two transcripts for the NCX1 and NCX3 isoforms; (ii) immunofluorescence experiments showed that native NCX1 and InsP 3 Rs are largely codistributed in discrete areas of the plasma membrane in close apposition to the cortical endoplasmic reticulum shell; (iii) when stimulated by rabbit serum, which elevates intracellular Ca 2+ mediated by InsP 3, voltage-clamped oocytes display a large and transient inward Ca 2+ -activated chloride current, I Cl(Ca), as a result of the Ca 2+ rise at the inner surface membrane; (iv) this current is significantly enhanced by KB-R7943 and by an extracellular sodium-depleted medium, two maneuvers that prevent “Ca 2+ extrusion” via NCX; and (v) blocking NCX enhanced the I Cl(Ca) elicited by InsP 3 but not by Ca 2+ photolysis in oocytes injected with the respective caged compounds. Moreover, overexpression of cardiac NCX1, confirmed by confocal microscopy, has functional consequences for the “Ca 2+ influx” but not for the serum-elicited “Ca 2+ efflux” mode of basal exchange activity and does not alter the number of endogenous NCX/InsP 3 Rs colocalization sites. Our results suggest that native NCX, because of its strategic position, may regulate InsP 3 -mediated Ca 2+ signaling during the early phases of oocyte maturation and/or fertilization, and furthermore foreign cardiac protein is excluded from the Ca 2+ microdomains surrounding the native NCX/InsP 3 Rs complex in the oocyte.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0021925820676876; http://dx.doi.org/10.1074/jbc.m408872200; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=10644229948&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/15375168; https://linkinghub.elsevier.com/retrieve/pii/S0021925820676876; http://www.jbc.org/lookup/doi/10.1074/jbc.M408872200; https://syndication.highwire.org/content/doi/10.1074/jbc.M408872200; https://dx.doi.org/10.1074/jbc.m408872200
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know