Gγ13 Interacts with PDZ Domain-containing Proteins *
Journal of Biological Chemistry, ISSN: 0021-9258, Vol: 281, Issue: 16, Page: 11066-11073
2006
- 28Citations
- 30Captures
- 3Mentions
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations28
- Citation Indexes28
- 28
- CrossRef20
- Captures30
- Readers30
- 30
- Mentions3
- References3
- 3
Article Description
The G protein γ13 subunit (Gγ13) is expressed in taste and retinal and neuronal tissues and plays a key role in taste transduction. We identified PSD95, Veli-2, and other PDZ domain-containing proteins as binding partners for Gγ13 by yeast two-hybrid and pull-down assays. In two-hybrid assays, Gγ13 interacted specifically with the third PDZ domain of PSD95, the sole PDZ domain of Veli-2, and the third PDZ domain of SAP97, a PSD95-related protein. Gγ13 did not interact with the other PDZ domains of PSD95. Coexpression of Gγ13 with its Gβ1 partner did not interfere with these two-hybrid interactions. The physical interaction of Gγ13 with PSD95 in the cellular milieu was confirmed in pull-down assays following heterologous expression in HEK293 cells. The interaction of Gγ13 with the PDZ domain of PSD95 was via the C-terminal C AAX tail of Gγ13 (where AA indicates the aliphatic amino acid); alanine substitution of the CTAL sequence at the C terminus of Gγ13 abolished its interactions with PSD95 in two-hybrid and pull-down assays. Veli-2 and SAP97 were identified in taste tissue and in Gγ13-expressing taste cells. Coimmunoprecipitation of Gγ13 and PSD95 from brain and of Gγ13 and SAP97 from taste tissue indicates that Gγ13 interacts with these proteins endogenously. This is the first demonstration that PDZ domain proteins interact with heterotrimeric G proteins via the C AAX tail of Gγ subunits. The interaction of Gγ13 with PDZ domain-containing proteins may provide a means to target particular Gβγ subunits to specific subcellular locations and/or macromolecular complexes involved in signaling pathways.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0021925819562834; http://dx.doi.org/10.1074/jbc.m600113200; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33744959425&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/16473877; http://www.jbc.org/lookup/doi/10.1074/jbc.M600113200; https://syndication.highwire.org/content/doi/10.1074/jbc.M600113200; https://linkinghub.elsevier.com/retrieve/pii/S0021925819562834; https://dx.doi.org/10.1074/jbc.m600113200
American Society for Biochemistry & Molecular Biology (ASBMB)
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know