Differential Modulation of 3T3-L1 Adipogenesis Mediated by 11β-Hydroxysteroid Dehydrogenase-1 Levels *
Journal of Biological Chemistry, ISSN: 0021-9258, Vol: 282, Issue: 15, Page: 11038-11046
2007
- 24Citations
- 18Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations24
- Citation Indexes24
- 24
- CrossRef21
- Captures18
- Readers18
- 18
Article Description
The localized activation of circulating glucocorticoids in vivo by the enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) plays a critical role in the development of the metabolic syndrome. However, the precise contribution of 11β-HSD1 in the initiation of adipogenesis by inactive glucocorticoids is not fully understood. 3T3-L1 fibroblasts can be terminally differentiated to mature adipocytes in a glucocorticoid-dependent manner. Both inactive rodent dehydrocorticosterone and human cortisone were able to substitute for the synthetic glucocorticoid dexamethasone in 3T3-L1 adipogenesis, suggesting a potential role for 11β-HSD1 in these effects. Differentiation of 3T3-L1 cells caused a strong increase in 11β-HSD1 protein levels, which occurred late in the differentiation protocol. Reduction of 11β-HSD1 activity in 3T3-L1 fibroblasts, achieved by pharmacological inhibition or adenovirally mediated delivery of short hairpin RNA constructs, specifically blocked the ability of inactive glucocorticoids to drive 3T3-L1 differentiation. However, even modest increases in exogenous 11β-HSD1 expression in 3T3-L1 fibroblasts, to levels comparable with endogenous 11β-HSD1 in differentiated 3T3-L1 adipocytes, were sufficient to block adipogenesis. Luciferase reporter assays indicated that overexpressed 11β-HSD1 was catalyzing the inactivating dehydrogenase reaction, because the ability of both active and inactive glucocorticoids to activate the glucocorticoid receptor were largely suppressed. These results suggest that the temporal regulation of 11β-HSD1 expression is tightly controlled in 3T3-L1 cells, so as to mediate the initiation of differentiation by inactive glucocorticoids and also to prevent the inhibitory activity of prematurely expressed 11β-HSD1 during adipogenesis.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0021925820766810; http://dx.doi.org/10.1074/jbc.m606197200; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34249743087&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/17311922; http://www.jbc.org/lookup/doi/10.1074/jbc.M606197200; https://syndication.highwire.org/content/doi/10.1074/jbc.M606197200; https://linkinghub.elsevier.com/retrieve/pii/S0021925820766810; https://dx.doi.org/10.1074/jbc.m606197200
American Society for Biochemistry & Molecular Biology (ASBMB)
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know