Recovery of free lutein from tagetes erecta: Determination of suitable saponification and crystallization conditions
Separation Science and Technology, ISSN: 0149-6395, Vol: 46, Issue: 2, Page: 265-271
2011
- 37Citations
- 41Captures
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Article Description
This study deals with the separation and purification of lutein from marigold flowers. The effects of saponification conditions such as time (0-7hr), volume to weight ratio of KOH solution to oleoresin (0.3 ml:1 g to 1 ml:1 g), and the concentration of KOH solution (25%-45% w/v) were first determined on the conversion of lutein fatty acid esters to free lutein. For 1 g of oleoresin, 0.5 ml of 40% v/v KOH solution was found to be the most suitable amount and concentration for the saponification of lutein esters to free lutein at 75°C. Following saponification, crystallization was carried out to determine the effect of crystallization solvents (water-ethanol and water-isopropanol mixtures), the composition of the crystallization solvents (2:0.3-2:1 v/v), and crystallization time (0.25-2 hr) on the yield and purity of free lutein. The results revealed that crystallization of the saponified sample in water-ethanol mixture at the volume ratio of 2:0.5 for 0.5 hr presented the highest yield of 99.12% of free lutein, at the purity of 85.53%. © Taylor & Francis Group, LLC.
Bibliographic Details
Informa UK Limited
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