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Novel glycosaminoglycan glycotechnology: Method for hybrid synthesis of glycosaminoglycan chains utilizing chemo-enzymatic procedures

Journal of Carbohydrate Chemistry, ISSN: 0732-8303, Vol: 29, Issue: 7, Page: 315-331
2010
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Article Description

A novel method for the synthesis of glycosaminoglycan chains is described. The 4,5-unsaturated hexuronic acid located at the nonreducing terminal, which derived from eliminase digestion of glycosaminoglycan, is chemically converted to glucuronic acid by employing the oxymercuration-demercuration reaction. Then, utilizing these oligosaccharides as acceptors for the transglycosylation reaction of bovine testicular hyaluronidase, glycosaminoglycan oligosaccharides were successfully constructed. It is thought that the present method will contribute to the development of glycosaminoglycan glycobiology and technology. Copyright © Taylor & Francis Group, LLC.

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