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Fluorescence Lifetime Imaging Microscopy (FLIM): Instrumentation and Applications

Critical Reviews in Analytical Chemistry, ISSN: 1547-6510, Vol: 23, Issue: 5, Page: 369-395
1992
  • 111
    Citations
  • 0
    Usage
  • 56
    Captures
  • 0
    Mentions
  • 0
    Social Media
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Metrics Details

  • Citations
    111
    • Citation Indexes
      111
  • Captures
    56

Article Description

The new and novel techniques of fluorescence lifetime imaging (FLI), and fluorescence lifetime imaging microscopy (FLIM) provide the investigator with the capacity to quantitate two-dimensional fluorescence intensity distributions and lifetimes. The concept, theory, and instrumentation of FLI and FLIM are reviewed in this paper. The implementation of FLIM instrumentation with conventional and confocal microscopic systems is discussed. These instruments permit the quantitative measurement of molecular interactions and chemical environment from samples in biological, physical, and environmental sciences. Numerous applications in the biomedical sciences for FLIM instrumentation are also discussed. © 1992, Taylor & Francis Group, LLC. All rights reserved.

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