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Trolox protection of myelin membrane in hydrogen peroxide-treated mature oligodendrocytes

Free Radical Research, ISSN: 1071-5762, Vol: 41, Issue: 4, Page: 444-451
2007
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Article Description

Oligodendrocytes have the highest rate of metabolic activity in the brain and are highly vulnerable to oxidative stress. In this work we determined the protective effect of Trolox, a water-soluble analogue of vitamin E, and insulin, a peptide shown to be neuroprotective, in oligodendrocyte lesion induced by hydrogen peroxide (H O). Exposure of primary cultures of rat oligodendrocytes to HO dose-dependently decreased their reducing capacity, as determined by the MTT assay. HO (100 μM) had no effect on Bax levels, active-caspase-3, DNA fragmentation or lactate dehydrogenase (LDH) leakage. Nevertheless, under these conditions, HO decreased the levels of myelin basic protein (MBP), used as a marker for oligodendrocyte myelin membrane. Treatment with insulin alone increased MBP levels, but no changes were observed in the presence of insulin plus H O. In contrast, incubation with Trolox completely prevented HO-induced decrease in MBP expression, suggesting that vitamin E analogues may prevent against oligodendrocyte oxidative damage.

Bibliographic Details

Perfeito, Rita; Pereira, Jorge; Oliveira, Catarina R; Bettencourt-Relvas, João; Rego, A Cristina

Informa UK Limited

Biochemistry, Genetics and Molecular Biology

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