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Enhancing effect of human thyroid stimulating hormone (hTSH) monoclonal antibody in the hTSH immunoradiometric assay (IRMA) system

Journal of Immunoassay and Immunochemistry, ISSN: 1532-1819, Vol: 33, Issue: 3, Page: 325-336
2012
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Article Description

Different monoclonal antibodies, both commercial and indigenously produced, were evaluated in various combinations to optimize an immunoradiometric assay (IRMA) system for human thyroid stimulating hormone (hTSH). During these studies, it was observed that mixing one of the indigenously produced hTSH monoclonal antibody (2B11) in the hTSH IRMA system using Immunotech (Beckman Coulter, Czech Republic) kit reagents, led to an overall increase in the assay binding and sensitivity (from 0.025 mIU/L to 0.015mIU/L) of this IRMA system. This is not a general property of all monoclonal antibodies against hTSH. The mechanism for this enhancement can be attributed to the formation of a multicomponent complex. © 2012 Copyright Taylor and Francis Group, LLC.

Bibliographic Details

Shubhangi Mirapurkar; G. Samuel; S. D. Borkute; N. Sivaprasad

Informa UK Limited

Medicine; Immunology and Microbiology; Biochemistry, Genetics and Molecular Biology; Health Professions

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