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Modified binary hammerhead ribozymes with high catalytic activity

Nucleosides, Nucleotides and Nucleic Acids, ISSN: 1525-7770, Vol: 24, Issue: 5-7, Page: 1105-1109
2005
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Conference Paper Description

A series of binary hammerhead ribozymes was designed and assessed in terms of cleavage activity and nuclease resistance. Enhanced nuclease resistance of binary ribozymes was achieved by incorporation of 2′-modified nucleotides at the selective positions along with addition of 3′-3′-linked thymidine cap. These modified binary ribozymes efficiently cleave 190-nucleotides long MDR1 mRNA fragment and display catalytic activity much higher then respective full-length analogs. Copyright © Taylor & Francis, Inc.

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