Phosphorylation of actopaxin regulates cell spreading and migration
Journal of Cell Biology, ISSN: 0021-9525, Vol: 166, Issue: 6, Page: 901-912
2004
- 45Citations
- 27Captures
- 1Mentions
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Metrics Details
- Citations45
- Citation Indexes45
- 45
- CrossRef39
- Captures27
- Readers27
- 27
- Mentions1
- References1
- Wikipedia1
Article Description
Actopaxin is an actin and paxillin binding protein that localizes to focal adhesions. It regulates cell spreading and is phosphorylated during mitosis. Herein, we identify a role for actopaxin phosphorylation in cell spreading and migration. Stable clones of U2OS cells expressing actopaxin wild-type (WT), nonphosphorylatable, and phosphomimetic mutants were developed to evaluate actopaxin function. All proteins targeted to focal adhesions, however the nonphosphorylatable mutant inhibited spreading whereas the phosphomimetic mutant cells spread more efficiently than WT cells. Endogenous and WT actopaxin, but not the nonphosphorylatable mutant, were phosphorylated in vivo during cell adhesion/spreading. Expression of the nonphosphorylatable actopaxin mutant significantly reduced cell migration, whereas expression of the phosphomimetic increased cell migration in scrape wound and Boyden chamber migration assays. In vitro kinase assays demonstrate that extracellular signal-regulated protein kinase phosphorylates actopaxin, and treatment of U2OS cells with the MEK1 inhibitor UO126 inhibited adhesion-induced phosphorylation of actopaxin and also inhibited cell migration.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=4544371915&origin=inward; http://dx.doi.org/10.1083/jcb.200404024; http://www.ncbi.nlm.nih.gov/pubmed/15353548; https://rupress.org/jcb/article/166/6/901/51260/Phosphorylation-of-actopaxin-regulates-cell; https://dx.doi.org/10.1083/jcb.200404024
Rockefeller University Press
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