The effect of feed supplement on growth, survival rate and immunity response of Pacific white shrimp Litopenaeus vannamei

An efforts to improve growth, survival and immunity response are still main issues in shrimp farming. The purpose of this study was to determine the effect of dietary supplements on the growth, survival and immunity response in order to increase phenotypic performance of Litopenaeus vannamei. The experiments were done by supplemented feed using i.e.,(A) Synthetic nucleotides (0.03 % of feed), (B) whole cell of Saccharomyces cerevisiae (100 mL/kg feed) and (C) Control (without feed supplement) and fed to the shrimp, Litopenaeus vannamei. The supplements were mixed in feed formulated and coated with chitosan, while the control without feed supplement. The shrimp larvae were reared in 5 m3 tanks with two replications The result showed that survival rate of shrimp that reared with supplemented synthetic nucleotide was 69,29% and with whole cell was 75,45% and control was 67,61%. Total haemocyte of shrimp before challenge test ranged from 287.2 to 465.7 x 10 cells/mL, but after challenge test with WSSV, have shown different number of cell densities where in the treatments of synthetic nucleotide was 303.63 x10cell/mL, whereas in whole cell and control were 265 x 10 cell / mL and 254,25 x 10 cell / mL respectively. Immunity response of L. vannamei shrimp expressed from ProPO was significantly different between supplemented feed compared to control after challenged with WSSV, TSV and IMNV.