PlumX Metrics
Embed PlumX Metrics

Molecular Cloning and Sequence Analysis of the cDNA Encoding Porcine Acrosin Inhibitor

DNA and Cell Biology, ISSN: 1557-7430, Vol: 13, Issue: 4, Page: 389-394
1994
  • 9
    Citations
  • 0
    Usage
  • 4
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

Article Description

A full-length cDNA encoding the porcine acrosin inhibitor has been isolated from a boar seminal vesicle cDNA library. Nucleotide sequence analysis of the 667-bp cDNA predicts a precursor protein of 97 amino acid residues, which includes a 26-residue signal peptide and a 71-residue secreted protein. The predicted amino acid sequence of the mature protein agrees completely with that of the sperm-associated acrosin inhibitor determined by conventional amino acid sequence analysis. However, the asparagine/aspartic acid and glutamine/glutamic acid substitutions, as reported in the seminal plasma counterpart, have not been observed. Southern blot analysis shows only a single hybridizing band with three different restriction endonucleases, suggesting the presence of a single copy of the acrosin inhibitor gene in the porcine genome. © 1994, Mary Ann Liebert, Inc. All rights reserved.

Bibliographic Details

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know