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Chromosome condensation factor Brn1p is required for chromatid separation in mitosis

Molecular Biology of the Cell, ISSN: 1059-1524, Vol: 11, Issue: 4, Page: 1305-1313
2000
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Article Description

This work describes BRN1, the budding yeast homologue of Drosophila Barren and Xenopus condensin subunit XCAP-H. The Drosophila protein is required for proper chromosome segregation in mitosis, and Xenopus protein functions in mitotic chromosome condensation. Mutant brn1 cells show a defect in mitotic chromosome condensation and sister chromatid separation and segregation in anaphase. Chromatid cohesion before anaphase is properly maintained in the mutants. Some brn1 mutant cells apparently arrest in S- phase, pointing to a possible function for Brn1p at this stage of the cell cycle. Brn1p is a nuclear protein with a nonuniform distribution pattern, and its level is up-regulated at mitosis. Temperature-sensitive mutations of BRN1 can be suppressed by overexpression of a novel gene YCG1, which is homologous to another Xenopus condensin subunit, XCAP-G. Overexpression of SMC2, a gene necessary for chromosome condensation, and a homologue of the XCAP-E condensin, does not suppress brn1, pointing to functional specialization of components of the condensin complex.

Bibliographic Details

Ilia I. Ouspenski; Olga A. Cabello; B. R. Brinkley; Tim Stearns

American Society for Cell Biology (ASCB)

Biochemistry, Genetics and Molecular Biology

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