Rapid and accurate detection of African swine fever virus by DNA endonuclease-targeted CRISPR trans reporter assay
Acta Biochimica et Biophysica Sinica, ISSN: 1745-7270, Vol: 52, Issue: 12, Page: 1413-1419
2020
- 19Citations
- 18Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations19
- Citation Indexes19
- 19
- CrossRef16
- Captures18
- Readers18
- 18
Article Description
The first case of African swine fever (ASF) outbreak in China was reported in a suburban pig farm in Shenyang in 2018. Since then, the rapid spread and extension of ASF has become the most serious threat for the swine industry. Therefore, rapid and accurate detection of African swine fever virus (ASFV) is essential to provide effective strategies to control the disease. In this study, we developed a rapid and accurate ASFV-detection method based on the DNA endonuclease-targeted CRISPR trans reporter (DETECTR) assay. By combining recombinase polymerase amplification with CRISPR-Cas12a proteins, the DETECTR assay demonstrated a minimum detection limit of eight copies with no cross reactivity with other swine viruses. Clinical blood samples were detected by DETECTR assay and showed 100% (30/30) agreement with real-time polymerase chain reaction assay. The rapid and accurate detection of ASFV may facilitate timely eradication measures and strict sanitary procedures to control and prevent the spread of ASF.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85099172552&origin=inward; http://dx.doi.org/10.1093/abbs/gmaa135; http://www.ncbi.nlm.nih.gov/pubmed/33201182; http://engine.scichina.com/doi/10.1093/abbs/gmaa135; https://dx.doi.org/10.1093/abbs/gmaa135; https://www.sciengine.com/ABBS/doi/10.1093/abbs/gmaa135; http://sciencechina.cn/gw.jsp?action=cited_outline.jsp&type=1&id=6885533&internal_id=6885533&from=elsevier
China Science Publishing & Media Ltd.
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