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Long noncoding RNA MNX1-AS1 functions as a competing endogenous RNA to regulate epithelial-mesenchymal transition by sponging MiR-744-5p in colorectal cancer

Bioscience, Biotechnology and Biochemistry, ISSN: 1347-6947, Vol: 85, Issue: 3, Page: 568-578
2021
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Article Description

Colorectal cancer (CRC) is the fourth most deadly cancer globally. Long noncoding RNA MNX1-AS1 has been proven to play a regulatory role in various human cancers. The present research aimed to explore the MNX1-AS1 function in CRC and the corresponding mechanism. A series of experiments were conducted to detect the effects of MNX1-AS1 and miR-744-5p on the biological function of CRC cells, including quantitative reverse transcription polymerase chain reaction, CCK-8, transwell, wound healing assay, Western blot, and dual-luciferase report assay. MNX1-AS1 was elevated in CRC tissues and cell lines. Si-MNX1-AS1 inhibited cell viability, invasion, migration, and the protein expressions of N-cadherin and Vimentin but promoted the protein expression of E-cadherin. MiR-744-5p bound to MNX1-AS1. MiR-744-5p inhibitor had the opposite effect of si-MNX1-AS1. Cotransfection of miR-744-5p inhibitor and si-MNX1-AS1 recovered the effects mentioned above. In conclusion, MNX1-AS1/miR-744-5p axis plays a pivotal role in the viability, invasion, migration, and epithelial-mesenchymal transition of colorectal cancer cells.

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