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Various penA mutations together with mtrR, porB and ponA mutations in Neisseria gonorrhoeae isolates with reduced susceptibility to cefixime or ceftriaxone

Journal of Antimicrobial Chemotherapy, ISSN: 0305-7453, Vol: 65, Issue: 4, Page: 669-675
2010
  • 85
    Citations
  • 0
    Usage
  • 78
    Captures
  • 1
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    85
  • Captures
    78
  • Mentions
    1
    • News Mentions
      1
      • News
        1

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Emergence and Spread of Cephalosporin-Resistant Neisseria gonorrhoeae with Mosaic penA Alleles, South Korea, 2012-2017.(SYNOPSIS)(Report)

Neisseria gonorrhoeae remains a major public health threat globally (1). During the past 2 decades, N. gonorrhoeae strains with resistance to extended-spectrum cephalosporins (ESCs), including

Article Description

Objectives: To examine mutations within the penA, mtrR, porB, ponA and pilQ genes of Neisseria gonorrhoeae to determine their contribution to cephalosporin resistance. Methods: A total of 46 N. gonorrhoeae isolates with reduced susceptibility to cefixime or ceftriaxone (MICs≥0.12 mg/L) and two susceptible isolates were selected. The full sequence of penA and partial sequences previously reported as hot mutation sites of the other genes were analysed. Genotyping by N. gonorrhoeae multiantigen sequence typing (NG-MAST) was also performed. Results: A mosaic penicillin-binding protein 2 (PBP 2) was found in a single isolate that exhibited the highest cefixime MIC (0.5 mg/L). The majority of the isolates with reduced susceptibility to cephalosporins contained non-mosaic PBP 2 sequences, of which PBP 2 pattern XIII was most common (28/46). All isolates with reduced susceptibility to cephalosporins also had mtrR and porB mutations. Two susceptible isolates had the PBP 2 pattern XIV and an incomplete MtrR protein, which was a new mutation. Isolates with identical PBP 2 patterns comprised multiple NG-MAST sequence types. Conclusions: Reduced susceptibility of N. gonorrhoeae to ceftriaxone and cefixime was associated with diverse penA mutations, particularly PBP 2 pattern XIII containing an Ala-501→Val substitution, together with mtrR and porB mutations. The existence of only one strain having the mosaic penA sequence indicated that ceftriaxone and cefixime resistance in Korea is mostly not associated with a mosaic penA sequence. Highly heterogeneous NG-MAST sequence types excluded the clonal expansion of a particular subtype. © The Author 2010.

Bibliographic Details

Lee, Sang-Guk; Lee, Hyukmin; Jeong, Seok Hoon; Yong, Dongeun; Chung, Gyung Tae; Lee, Yeong Seon; Chong, Yunsop; Lee, Kyungwon

Oxford University Press (OUP)

Pharmacology, Toxicology and Pharmaceutics; Medicine

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