25-Hydroxyvitamin D 1α- and 24-Hydroxylase Activities in Pig Kidney Homogenates: Effect of Vitamin D Deficiency
The Journal of Nutrition, ISSN: 0022-3166, Vol: 114, Issue: 1, Page: 119-126
1984
- 24Citations
- 11Captures
Metric Options: CountsSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations24
- Citation Indexes24
- 24
- CrossRef20
- Captures11
- Readers11
- 11
Article Description
A method was developed for simultaneous assay of 25-hydroxyvitamin D 1α- and 24-hydroxylase in pig kidney homogenates. The products of these enzymes, 1,25-dihydroxycholecalciferol and 24,25-dihydroxycholecalciferol, were extracted from the in vitro incubation mixtures, isolated and purified by gel filtration and high-performance liquid chromatography, identified by ultraviolet absorption spectrometry, and high performance liquid chromatography with a second solvent system and quantified relative to authentic standards with a 254-nm detector system. Assay conditions included 12.5 μM substrate (25-hydroxycholecalciferol) concentration and an incubation time of 15 minutes at 37°C, which was on the linear portion of time curves for both enzymes. Maximum enzyme velocity was 1548 pmol/minute per gram kidney tissue for 1α-hydroxylase and 286 pmol/minute per gram kidney tissue for 24-hydroxylase. The apparent K m for pig kidney 25-hydroxyvitamin D 1α-hydroxylase (1α-hydroxylase) was 445 nM and for 25-hydroxyvitamin D 24-hydroxylase (24-hydroxylase) was 833 nM. We also demonstrated the use of this assay in pigs fed a semisynthetic diet with or without vitamin D. Pigs fed vitamin D-deficient diet had a 5- to 10-fold increase in 1α-hydroxylase activity, severe hypocalcemia, low plasma 1,25-dihydroxycholecalciferol, very low plasma (undetectable) 24,25-dihydroxy-cholecalciferol concentration, and no detectable 24-hydroxylase activity compared to those fed the vitamin D-replete diet.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0022316623199299; http://dx.doi.org/10.1093/jn/114.1.119; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0021331323&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/6546396; https://linkinghub.elsevier.com/retrieve/pii/S0022316623199299; https://dx.doi.org/10.1093/jn/114.1.119
Elsevier BV
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know