Silencing of a plant gene by transcriptional interference
Nucleic Acids Research, ISSN: 0305-1048, Vol: 37, Issue: 11, Page: 3739-3746
2009
- 17Citations
- 52Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations17
- Citation Indexes17
- 17
- CrossRef8
- Captures52
- Readers52
- 52
Article Description
Integration of foreign DNA into eukaryotic genomes results frequently in a total or partial loss of gene function, caused by the interruption of indispensable structures of the gene itself. Using T-DNA insertions in Arabidopsis we screened for mutants with deregulated chlorophyll precursor accumulation in etiolated seedlings. A mutant designated rfd1 (red fluorescent in darkness) with increased protochlorophyllide accumulation showed a fluorescent phenotype that was associated with a lack of transcript initiation from the At RibA1 promoter situated downstream of the integrated T-DNA. Complementation experiments confirmed rfd1 to be a knockout phenotype. Comparison with two SALK insertion lines bearing T-DNA integrations in the 5′UTR of At RibA1 demonstrated that the insertion event in rfd1 itself does not explain the complete lack of transcript initiation. A 35S tetrameric enhancer sequence present on the rfd1 T-DNA causes the overaccumulation of a large polycistronic transcript originating inside the T-DNA. This 5.5-kb RNA runs over the downstream situated At RibA1 promoter, which was shown by 5′RACE analyses to be consequently silenced. Hence, a transcription process that starts upstream and overlaps At RibA1 blocks the initiation at the At RibA1 promoter in rfd1. This regulatory mechanism has recently been introduced in yeast as transcriptional interference and is described here for the first time in a plant system. © 2009 The Author(s).
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=67649871122&origin=inward; http://dx.doi.org/10.1093/nar/gkp241; http://www.ncbi.nlm.nih.gov/pubmed/19376832; https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gkp241; https://dx.doi.org/10.1093/nar/gkp241; https://academic.oup.com/nar/article/37/11/3739/1092114
Oxford University Press (OUP)
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