Detection of HLA-specific IGG antibodies using single recombinant HLA alleles: The monoLISA assay
Transplantation, ISSN: 0041-1337, Vol: 70, Issue: 3, Page: 531-536
2000
- 14Citations
- 2Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations14
- Citation Indexes14
- 14
- CrossRef13
- Captures2
- Readers2
Article Description
Background. Because of the presence of confounding antigens, the assignment of HLA antibody specificity is difficult in highly sensitized patients, and the definition of an acceptable HLA mismatch requires a significant workload per patient. We describe a new ELISA method, monoLISA, for detection of immuno-globulin (Ig)G HLA antibody using single recombinant HLA class I monomers bound to microtiter plates. Methods. HLA-A2 and -B8 monomers were synthesized and used as screening targets for 85 sera from renal patients. The sera contained various IgG and IgM HLA-specific antibodies, including anti-A2 and anti-B8, defined in a conventional complement-dependent cytotoxicity test (CDC). Investigations were performed to determine possible effects on antibody binding of differential monomer peptide presentation as well as lack of glycosylation. Results. A good correlation was found between CDC-defined specificities and the reactivity observed with HLA monomers. MonoLISA attained means of 100% sensitivity and 92.5% specificity compared with CDC. Neither the presence of different peptides, nor the absence of glycosylation of the monomer affected the ability of monoLISA to detect antibody. Conclusion. This study demonstrates that the MonoLISA method for HLA antibody detection is valid. Because this has the potential to reduce the work involved in screening sensitized patients awaiting transplantation for HLA antibodies, resources aimed at increasing the number of constructed monomers would be well targeted.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0034663789&origin=inward; http://dx.doi.org/10.1097/00007890-200008150-00023; http://www.ncbi.nlm.nih.gov/pubmed/10949199; http://journals.lww.com/00007890-200008150-00023; https://dx.doi.org/10.1097/00007890-200008150-00023; https://journals.lww.com/transplantjournal/Fulltext/2000/08150/DETECTION_OF_HLA_SPECIFIC_IGG_ANTIBODIES_USING.23.aspx
Ovid Technologies (Wolters Kluwer Health)
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