LPS abolishes extrasplenic vasoconstriction to atrial natriuretic peptide: The role of NO and endothelin 1

Sepsis causes changes in vascular resistance and hypovolemia. Previous studies have demonstrated that the spleen regulates blood volume via atrial natiuretic peptide (ANP). We hypothesized that LPS alters extrasplenic responses to ANP via endothelial-dependent mechanisms and studied the role of NO and endothelin 1 (ET-1). Isolated extrasplenic arteries and veins (vessels in mesentery adjoining spleen) were obtained from male Wistar rats weighing 200 to 280 g (n = 102) and mounted on a pressure myograph to determine intraluminal diameter for 4 h. Isolated vessels constricted in response to the half-maximum response of ANP (veins, 30% ± 1.7%; arteries, 34.5 ± 1.7%; P < 0.05), and this was abolished by the NO donor S-nitroso-N-acetylpenicillamine (SNAP 75 μM). Arteries and veins incubated with LPS (50 μg mL for 4 h) were unresponsive to ANP, and constriction was not restored by the NOS inhibitor Nω-nitro-L-arginine methyl ester (L-NAME 100 μM). However, venular constriction returned in the presence of the ET-1 antagonist Bosentan, increasing from -1.5 ± 1.2 (10 min) to -10 ± 2.5% (4 h) with LPS + Bosentan (3 × 10 M) compared with -2.3 ± 1.2 and 0% with LPS alone. In conclusion, LPS abolished endothelial-dependent extrasplenic venular constriction to ANP partially due to increased ET-1, whereas NO seemed to modulate vascular responses to ANP. ©2008The Shock Society.