GABAergic projections from the subplate to Cajal-Retzius cells in the neocortex

Subplate neurons and Cajal-Retzius cells play an important role in the corticogenesis. Despite morphological evidence, the question whether subplate neurons innervate Cajal-Retzius cells has not been studied yet. We report that electrical stimulation in the subplate resulted in evoked GABAergic inhibitory postsynaptic currents (eIPSCs) in Cajal-Retzius cells. The eIPSC latency showed minor variability and amounted to approximately 4 ms, suggesting the monosynaptic connection. During the first postnatal week: (i) eIPSC amplitude increased, (ii) eIPSC kinetics sped up, (iii) the size of readily releasable pool increased, and (iv) γ-aminobutyric acid release probability decreased. We conclude that GABAergic subplate neurons innervate Cajal-Retzius cells. Surprisingly, despite the transient nature of both cell populations, these projections show developmental adjustments typical for many nontransient synaptic connections. © 2011 Wolters Kluwer Health | Lippincott Williams &Wilkins.