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Hwanghaeicola aestuarii gen. nov., sp. nov., a moderately halophilic bacterium isolated from a tidal flat of the Yellow Sea

International Journal of Systematic and Evolutionary Microbiology, ISSN: 1466-5026, Vol: 60, Issue: 12, Page: 2877-2881
2010
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A moderately halophilic Gram-staining-negative bacterium, designated strain Y26, was isolated from a tidal flat of Taean coast in South Korea. Cells were strictly aerobic, motile cocci with a single flagellum and showed catalase- and oxidase-positive reactions. Growth of strain Y26 was observed at 15-35°C (optimum 25-30°C), pH 6.0-8.0 (optimum pH 6.5-7.5) and with 1.5-6.0% (w/v) NaCl (optimum 2.0-3.0 %). The predominant fatty acids were Cω7c (66.2 %), C (12.4 %) and C 3-OH (5.0 %) and the G+C content of the genomic DNA was 61.0 mol%. Strain Y26 contained ubiquinone-10 (Q-10) as the major respiratory quinone. Comparative 16S rRNA gene sequence analysis showed that strain Y26 formed a distinct phyletic lineage from other genera within the Roseobacter clade of the class Alphaproteobacteria and was most closely related to members of the genera Maribius, Maritimibacter and Palleronia with 93.8-94.6% sequence similarity. On the basis of chemotaxonomic data and molecular properties, strain Y26 represents a novel genus, Hwanghaeicola, within the family Rhodobacteraceae, for which the name Hwanghaeicola aestuarii gen. nov., sp. nov. is proposed. The type strain is Y26 (=KACC 13705 =DSM 22009). © 2010 IUMS.

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