Coprobacter fastidiosus gen. nov., sp. nov., a novel member of the family Porphyromonadaceae isolated from infant faeces

A novel obligately anaerobic, non-spore-forming, rod-shaped, non-motile Gram-reaction-negative bacterium was isolated from infant faeces. The strain, designated NSB1, was able to grow on rich media at 30-37 °C, in the presence of up to 2% (w/v) Oxgall and 2% (w/v) NaCl. Cells of strain NSB1 produced catalase, but not urease and indole. Aesculin was not hydrolysed. The strain was able to utilize D-glucose, lactose, maltose, mannose and raffinose as electron donors. When grown on D-glucose, the main metabolic end products were propionic and acetic acids, with a minor product being succinic acid. The major cellular fatty acids, iso-C and anteiso-C, were present at a 1: 1 molar ratio. The major menaquinone was MK-11. The DNA G+C content was found to be 38.5 mol%. According to 16S rRNA gene sequence analysis strain NSB1 is a member of the family Porphyromonadaceae, phylum Bacteroidetes. The closest relatives of the strain were Barnesiella viscericola (88.2% identity) and Barnesiella intestinihominis (87.4% identity). On the basis of phenotypic and genotypic properties of strain NSB1 we conclude that this strain represent a novel species in a new genus within the family of Porphyromonadaceae for which the name Coprobacter fastidiosus gen. nov., sp. nov. is proposed. The type strain of the species is NSB1 (5DSM 26242,5VKM B-2743). © 2013 IUMS.