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Novosphingobium malaysiense sp. nov. isolated from mangrove sediment

International Journal of Systematic and Evolutionary Microbiology, ISSN: 1466-5026, Vol: 64, Issue: PART 4, Page: 1194-1201
2014
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Article Description

A novel bacterium, strain MUSC 273, was isolated from mangrove sediments of the Tanjung Lumpur river in the state of Pahang in peninsular Malaysia. The bacterium was yellow-pigmented, Gram-negative, rod-shaped and non-spore-forming. The taxonomy of strain MUSC 273 was studied by a polyphasic approach and the organism showed a range of phenotypic and chemotaxonomic properties consistent with those of the genus Novosphingobium. The 16S rRNA gene sequence of strain MUSC 273 showed the highest sequence similarity to those of Novosphingobium indicum H25 (96.8 %), N. naphthalenivorans TUT562 (96.4 %) and N. soli CC-TPE-1 (95.9 %) and lower sequence similarity to members of all other species of the genus Novosphingobium. Furthermore, in phylogenetic analyses based on the 16S rRNA gene sequence, strain MUSC 273 formed a distinct cluster with members of the genus Novosphingobium. DNA-DNA relatedness of strain MUSC 273 to the type strains of the most closely related species, N. indicum MCCC 1A01080 and N. naphthalenivorans DSM 18518, was 29.2 % (reciprocal 31.0 %) and 17 % (reciprocal 18 %), respectively. The major respiratory quinone was ubiquinone Q-10, the major polyamine was spermidine and the DNA G+C content was 63.3±0.1 mol%. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidyldimethylethanolamine, phosphatidylcholine and sphingoglycolipid. The major fatty acids were Cω7c, Cω6c, C, C 2-OH and Cω7c. Comparison of BOX-PCR fingerprints indicated that strain MUSC 273 represented a unique DNA profile. The combined genotypic and phenotypic data showed that strain MUSC 273 represents a novel species of the genus Novosphingobium, for which the name Novosphingobium malaysiense sp. nov. is proposed. The type strain is MUSC 273 (= DSM 27798 = MCCC 1A00645 = NBRC 109947). © 2014 IUMS.

Bibliographic Details

Lee, Learn-Han; Azman, Adzzie-Shazleen; Zainal, Nurullhudda; Eng, Shu-Kee; Fang, Chee-Mun; Hong, Kui; Chan, Kok-Gan

Microbiology Society

Immunology and Microbiology; Agricultural and Biological Sciences

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