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FluidFM as a Tool to Study Adhesion Forces of Bacteria - Optimization of Parameters and Comparison to Conventional Bacterial Probe Scanning Force Spectroscopy

bioRxiv, ISSN: 2692-8205
2019
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Article Description

The FluidFM enables the immobilization of single cells on a hollow cantilever using relative underpressure. In this study, we systematically optimize versatile measurement parameters to improve the quality of force-distance curves recorded with a FluidFM. Using single bacterial cells, we show that Single Cell Force Spectroscopy experiments with the FluidFM lead to comparable results to a conventional Single Cell Force Spectroscopy approach using polydopamine for chemical fixation of a bacterial cell.

Bibliographic Details

Hofherr, Linda; Müller-Renno, Christine; Ziegler, Christiane

Cold Spring Harbor Laboratory

Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Immunology and Microbiology; Neuroscience; Pharmacology, Toxicology and Pharmaceutics

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