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Transcripts of vaccinia virus postreplicative genes do not contain a 5' methylguanosine cap

bioRxiv, ISSN: 2692-8205
2020
  • 3
    Citations
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Metrics Details

  • Citations
    3
    • Citation Indexes
      3
      • CrossRef
        3

Article Description

Vaccinia virus (VACV) is a prototypical poxvirus originally used for eradication of smallpox. Investigation into VACV mRNAs carried out almost half a century ago substantially contributed to the fundamental discovery of the 5' mRNA cap, a hallmark of all eukaryotic and many viral mRNAs. VACV research also facilitated the identification and understanding of the general mechanism of 5' mRNA cap synthesis. We characterized the VACV transcripts at the individual mRNA molecule level and found that vaccinia postreplicative mRNAs, containing nontemplated 5' poly(A) leaders, surprisingly lack the 5' cap structure in vivo. We also show that the lengths of the nontemplated leaders and the presence or absence of cap structures at the 5' mRNA ends are controlled by the initiator sequence of the VACV postreplicative promoters.

Bibliographic Details

Václav Vopálenský; Michal Sýkora; Tomáš Mašek; Martin Pospíšek; Zora Mělková

Cold Spring Harbor Laboratory

Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Immunology and Microbiology; Neuroscience; Pharmacology, Toxicology and Pharmaceutics

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