PlumX Metrics
Embed PlumX Metrics

Glucose uptake in mammalian cells measured by ICP-MS

bioRxiv, ISSN: 2692-8205
2021
  • 0
    Citations
  • 0
    Usage
  • 0
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Article Description

We developed a sensitive, ratiometric method to measure simultaneously C-labeled glucose and rubidium in biological samples using ICP-MS. The method uses probe-assisted ultra-sonication with water to extract C-[6C]-labeled-D-glucose and other polar analytes from mammalian tissues. It extracts >80% of the reference value for Rb and >95 % of C in a CRM spiked with C-[6C]-labeled-D-glucose in the micro-molar range. Using optimized instrument conditions, the method achieves a stable C/C signal without spectral interferences. The C/C signal is independent of sample composition and depends linearly on the concentration of C-[6C]-labeled-D-glucose in spiked samples. Overall, the method achieves a limit of detection of 10 µM for 6-C-labeled C glucose in biological tissues. This detection capability for carbon in biological matrices by ICP-MS opens a wider range of applications for ICP-MS in biomedical research. As proof-of-principle, we combined C detection with the multi-channel capability of ICP-MS to measure glucose and rubidium uptake in the same contracting skeletal muscles. Multi-isotope detection is needed to study many biological processes, including coupled membrane transport. These results demonstrate a capability for carbon detection by ICP-MS that can significantly advance studies of complex biological processes that require multi-isotope detection.

Bibliographic Details

Natalie J. Norman; Tatiana L. Radzyukevich; Judith A. Heiny; Julio Landero-Figueroa; Joyce Ghali

Cold Spring Harbor Laboratory

Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Immunology and Microbiology; Neuroscience; Pharmacology, Toxicology and Pharmaceutics

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know