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Suppression of transposon mobilization by mA-mediated RNA sequestration in stress granules

bioRxiv, ISSN: 2692-8205
2022
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Article Description

Transposon is a mobile and ubiquitous DNA that can vastly causes genomic alterations. In plants, it is well documented that transposon mobilization is strongly repressed by DNA methylation; however, the roles of RNA methylation in transposon control remain unknown. Here we suggest that transposon RNA is marked by mA RNA methylation and is sequestered in stress granule (SG) in mA-dependent manner. Intriguingly, a SG-localized AtALKBH9B selectively demethylates a heat-activated retroelement Onsen, and thereby releases from spatial confinement allowing for its mobilization. In addition, we show evidence that mA RNA methylation contributes to transpositional suppression by inhibiting the virus-like particles assembly and extrachromosomal DNA production. In summary, this study unveils a hidden role for mA in the suppression of transposon mobility and provides an insight into how transposon counteracts the host’s epitranscriptomic control by hitchhiking RNA demethylase.

Bibliographic Details

Wenwen Fan; Ling Wang; Zhen Lei; Jie Chu; Jungnam Cho

Cold Spring Harbor Laboratory

Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Immunology and Microbiology; Neuroscience; Pharmacology, Toxicology and Pharmaceutics

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