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Endosomolytic Peptides Enable the Cellular Delivery of Peptide Nucleic Acids

bioRxiv, ISSN: 2692-8205
2024
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  • 5
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  • 1
    Mentions
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Metric Options:   Counts1 Year3 Year

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  • Captures
    5
  • Mentions
    1
    • News Mentions
      1
      • 1

Most Recent News

Endosomolytic Peptides Enable the Cellular Delivery of Peptide Nucleic Acids

2024 JUL 02 (NewsRx) -- By a News Reporter-Staff News Editor at Gene Therapy Daily News -- According to news reporting based on a preprint

Article Description

Precision genetic medicine enlists antisense oligonucleotides (ASOs) to bind to nucleic acid targets important for human disease. Peptide nucleic acids (PNAs) have many desirable attributes as ASOs but lack cellular permeability. Here, we use an assay based on the corrective splicing of an mRNA to assess the ability of synthetic peptides to deliver a functional PNA into a human cell. We find that the endosomolytic peptides L17E and L17ER4 are highly efficacious delivery vehicles. Co-treatment of a PNA with low micromolar L17E or L17ER4 enables robust corrective splicing in nearly all treated cells. Peptide–PNA conjugates are even more effective. These results enhance the utility of PNAs as research tools and potential therapeutic agents.

Bibliographic Details

Jo Lynn B. Giancola; Ronald T. Raines

Cold Spring Harbor Laboratory

Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Immunology and Microbiology; Neuroscience; Pharmacology, Toxicology and Pharmaceutics

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