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A legacy role for DNA binding of Lon protects against genotoxic stress

bioRxiv, ISSN: 2692-8205
2018
  • 10
    Citations
  • 0
    Usage
  • 0
    Captures
  • 1
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    10
    • Citation Indexes
      10
      • CrossRef
        10
  • Mentions
    1
    • News Mentions
      1
      • 1

Most Recent News

A legacy role for DNA binding of Lon protects against genotoxic stress

2023 JAN 05 (NewsRx) -- By a News Reporter-Staff News Editor at NewsRx Life Science Daily -- According to news reporting based on a preprint

Article Description

DNA binding proteins are essential for cellular life, but persistently bound complexes have toxic consequences. Here we show that the proteotoxic responsive bacterial protease Lon clears proteins from DNA to promote genotoxic stress resistance. Purified Lon binds DNA and degrades neighboring bound proteins, while a fully active DNA-blind Lon variant does not. This variant can degrade substrates as normal during unstressed growth, complements pleotropic phenotypes of ∆lon, including proteotoxic resilience, but remains sensitive to genotoxic stresses and fails to degrade proteins efficiently during DNA damage. Transposon sequencing reveals that ∆lon is vulnerable to loss of protein-DNA eviction factors and we use dynamic nucleoid occupancy profiling to show that chromosome-wide protein turnover relies on Lon DNA binding. Finally, disrupting Lon binding to mitochondria genomes also results in genotoxic stress sensitivity, consistent with the bacterial ancestry of this organelle. We propose that clearance of persistent proteins from DNA by Lon originated in free-living aproteobacteria and maintained during the evolution of mitochondria.

Bibliographic Details

Rilee D. Zeinert; Justyne L. Ogdahl; Jing Liu; Benjamin B. Barros; Peter Chien; Qiyuan Yang; Yunguang Du; Cole M. Haynes; Peter L. Freddolino

Cold Spring Harbor Laboratory

Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Immunology and Microbiology; Neuroscience; Pharmacology, Toxicology and Pharmaceutics

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