Influence of salt stress on the flg22 induced ROS production in arabidopsis thaliana leaves

In their natural habitats, plants have to cope with multiple stress factors triggering respective response pathways, leading to mutual interference. Our work aimed to study the effect of salt stress in combination with immune response triggered by microbe-associated molecular pattern (MAMP) in Arabidopsis thaliana Col-0 plants. We measured ROS production after treatment with flg22 and the influence of concomitant salt stress (NaCl and NaCO).The maximum combined effect of NaCl solution and flg22 on ROS production was achieved at 6 mM salt, which was almost 2 times higher than the single effect of MAMP. A similar maximum combined effect with NaCO was observed at 10 mM concentration. High concentration of NaCl and NaCO was accompanied with declining of ROS production, which was completely inhibited at 150 mM of NaCl and at 50 mM of NaCO.The immediate and long term (24 h) effect of NaCl on leaf tissue of Arabidopsis thaliana showed that the impact of salt stress on flg22induced ROS production probably did not affect the genetic aspects of the tissue response, but was associated with ionic and osmotic stresses. Experiments with mannitol, KCl and CaCl allowed to conclude that the observed effect was due to the ionic stress of the salt rather than the osmotic one.