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Labeling the components of the plasma membrane with fluorescent dyes for imaging

Cold Spring Harbor Protocols, ISSN: 1559-6095, Vol: 3, Issue: 2, Page: pdb.prot4930
2008
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INTRODUCTIONThe eukaryotic cell has evolved to compartmentalize its functions and transport various metabolites among cellular compartments. Therefore, in cell biology, the study of organization and structure/function relationships are of great importance. The plasma membrane provides the essential dynamic boundary and a vital selective interface between the cell compartment and its environment. This article describes four methods for labeling the plasma membrane with fluorescent probes. Each method labels a distinct class of membrane molecules.

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