Enrichment of Human Epidermal Langerhans Cells by Attachment to Erythrocyte Monolayers
Journal of Investigative Dermatology, ISSN: 0022-202X, Vol: 86, Issue: 1, Page: 9-12
1986
- 16Citations
- 1Captures
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Article Description
The present investigation introduces a method for purification of human epidermal Langerhans cells (LC). The method is based on the attachment of LC to IgG-coated sheep erythrocyte monolayers via their Fc receptors. To optimize the enrichment assay, several variables were tested. The best results were obtained when epidermal cells were centrifuged against erythrocyte monolayers; the purification procedure was performed at 4°C in the presence of 5% fetal calf serum, using about 6 × 10 6 epidermal cells per erythrocyte plate (diameter 5 cm). The average purity of the recovered LC was 80.9% and LC-depleted fractions contained an average of 0.5% DR-positive cells. LC were able to enhance significantly leukoagglutinin- and purified protein derivative-induced T lymphocyte proliferation and leukocyte migration inhibitory factor production.
Bibliographic Details
http://www.sciencedirect.com/science/article/pii/S0022202X86900898; http://dx.doi.org/10.1111/1523-1747.ep12283693; http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0022631990&origin=inward; http://www.ncbi.nlm.nih.gov/pubmed/3528306; https://linkinghub.elsevier.com/retrieve/pii/S0022202X86900898; https://dx.doi.org/10.1111/1523-1747.ep12283693
Elsevier BV
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