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Enrichment of Human Epidermal Langerhans Cells by Attachment to Erythrocyte Monolayers

Journal of Investigative Dermatology, ISSN: 0022-202X, Vol: 86, Issue: 1, Page: 9-12
1986
  • 16
    Citations
  • 0
    Usage
  • 1
    Captures
  • 0
    Mentions
  • 0
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Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    16
    • Citation Indexes
      16
  • Captures
    1

Article Description

The present investigation introduces a method for purification of human epidermal Langerhans cells (LC). The method is based on the attachment of LC to IgG-coated sheep erythrocyte monolayers via their Fc receptors. To optimize the enrichment assay, several variables were tested. The best results were obtained when epidermal cells were centrifuged against erythrocyte monolayers; the purification procedure was performed at 4°C in the presence of 5% fetal calf serum, using about 6 × 10 6 epidermal cells per erythrocyte plate (diameter 5 cm). The average purity of the recovered LC was 80.9% and LC-depleted fractions contained an average of 0.5% DR-positive cells. LC were able to enhance significantly leukoagglutinin- and purified protein derivative-induced T lymphocyte proliferation and leukocyte migration inhibitory factor production.

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