Induced autoprocessing of the cytopathic Makes caterpillars floppy-like effector domain of the Vibrio vulnificusMARTX toxin
Cellular Microbiology, ISSN: 1462-5822, Vol: 17, Issue: 10, Page: 1494-1509
2015
- 22Citations
- 21Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations22
- Citation Indexes22
- 22
- CrossRef13
- Captures21
- Readers21
- 21
Article Description
The multifunctional-autoprocessing repeats-in-toxin (MARTX) toxin that harbours a varied repertoire of effector domains is the primary virulence factor of Vibrio vulnificus. Although ubiquitously present among Biotype I toxin variants, the 'Makes caterpillars floppy-like' effector domain (MCF) is previously unstudied. Using transient expression and protein delivery, MCF and MCF from the Aeromonas hydrophilaMARTX toxin are shown for the first time to induce cell rounding. Alanine mutagenesis across the C-terminal subdomain of MCF identified an Arg-Cys-Asp (RCD) tripeptide motif shown to comprise a cysteine protease catalytic site essential for autoprocessing of MCF. The autoprocessing could be recapitulated in vitro by the addition of host cell lysate to recombinant MCF, indicating induced autoprocessing by cellular factors. The RCD motif is also essential for cytopathicity, suggesting autoprocessing is essential first to activate the toxin and then to process a cellular target protein resulting in cell rounding. Sequence homology places MCF within the C58 cysteine protease family that includes the type III secretion effectors YopT from Yersinia spp. and AvrPphB from Pseudomonas syringae. However, the catalytic site RCD motif is unique compared with other C58 peptidases and is here proposed to represent a new subgroup of autopeptidase found within a number of putative large bacterial toxins.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84942193827&origin=inward; http://dx.doi.org/10.1111/cmi.12451; http://www.ncbi.nlm.nih.gov/pubmed/25912102; https://onlinelibrary.wiley.com/doi/10.1111/cmi.12451; http://doi.wiley.com/10.1111/cmi.12451; http://onlinelibrary.wiley.com/doi/10.1111/cmi.12451/abstract
Hindawi Limited
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