PlumX Metrics
Embed PlumX Metrics

Design and synthesis of C-labelled proteins as tools for protein digestion studies in fish larvae

Aquaculture Nutrition, ISSN: 1353-5773, Vol: 11, Issue: 6, Page: 395-401
2005
  • 7
    Citations
  • 0
    Usage
  • 11
    Captures
  • 0
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    7
    • Citation Indexes
      7
  • Captures
    11

Article Description

In order to study the protein digestive capability in early stages of fish larvae, a method was developed to produce adequate amounts of pure, well-defined, high-quality, specifically C-labelled proteins. The proteins that were chosen were integral parts of the abundant cytoskeleton protein spectrin. The proteins were engineered by molecular biology technology to allow the specific incorporation of C-labelled leucine. The purified monodisperse proteins could be concentrated to more than 10 mg mL without precipitation. These proteins were parts of the same 'mother protein' with similar amino acid compositions and sequences but different molecular weights. This made them suitable to study the influence of the molecular sizes of the proteins on digestion in fish larvae. © 2005 Blackwell Publishing Ltd.

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know