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The expression of human plasma cholesteryl‐ester‐transfer protein in HepG2 cells in induced by sodium butyrate: Quantification of low mRNA levels by polymerase chain reaction

European Journal of Biochemistry, ISSN: 1432-1033, Vol: 218, Issue: 3, Page: 945-950
1993
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Article Description

Although human plasma cholesteryl‐ester‐transfer protein (CETP) is primarily synthesized in the liver, its expression in a number of transformed liver cell lines is very low. However the use of the human hepatoma cell line HepG2 as a model system for the regulation of CETP on mRNA level is facilitated by a quantitative reverse‐transcribed polymerase chain reaction. We demonstrate a time‐dependent and concentration‐dependent 3–4‐fold induction of CETP mRNA by sodium butyrate. CETP mass in the medium is also augmented; however, the effect on protein level is less pronounced. Copyright © 1993, Wiley Blackwell. All rights reserved

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