An overexpression screen in Saccharomyces cerevisiae identifies novel genes that affect endocytic protein trafficking
Traffic, ISSN: 1398-9219, Vol: 12, Issue: 11, Page: 1592-1603
2011
- 25Citations
- 85Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations25
- Citation Indexes25
- 25
- CrossRef24
- Captures85
- Readers85
- 85
Article Description
A large number of proteins involved in the biogenesis of yeast endosomes and vacuoles have been identified based on screens that scored for inactivation of proteins. Such screens may, however, miss important regulators of the pathway. Here, we present a visual screen in which we examined the effects on vacuole morphology if any of the 6153 yeast open reading frames was overexpressed. Using a progressive screening procedure, we could identify a total of 53 genes. Among the most striking endosomal proteins are the CORVET/HOPS subunits Vps3, Vps18 and Vps39 and the putative tethering inhibitor Ivy1. Furthermore, six endosomal sorting complex related to transport (ESCRT) proteins led to altered vacuole morphology if overproduced. Among the novel proteins, we identify Yer128w as an endosomal protein that interacts with the AAA-ATPase Vps4, and therefore named it Vfa1 (Vps Four-Associated 1). We present evidence on the possible role of these novel proteins in trafficking to the vacuole. Our data provide novel insights into the regulation of protein trafficking. © 2011 John Wiley & Sons A/S.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=80053578496&origin=inward; http://dx.doi.org/10.1111/j.1600-0854.2011.01252.x; http://www.ncbi.nlm.nih.gov/pubmed/21777356; https://onlinelibrary.wiley.com/doi/10.1111/j.1600-0854.2011.01252.x; https://dx.doi.org/10.1111/j.1600-0854.2011.01252.x
Wiley
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