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Campylobacter and Salmonella are prevalent in broiler farms in Kyushu, Japan: Results of a 2-year distribution and circulation dynamics audit

Journal of Applied Microbiology, ISSN: 1365-2672, Vol: 120, Issue: 6, Page: 1711-1722
2016
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Article Description

Aim: To elucidate the distribution and circulation dynamics of Campylobacter and Salmonella in Japanese chicken broiler flocks. Methods and Results: A 2-year investigation of the distribution of Campylobacter and Salmonella was conducted in 25 broiler flocks at nine farms in Japan from 2013 to 2014. Campylobacter and Salmonella tested positive in 11 (44·0%) and 24 (96·0%) broiler flocks respectively. One hundred and ninety-five Campylobacter and 184 Salmonella isolates were characterized into 12 Campylobacter (including two novel genotypes) and three Salmonella MLST genotypes. Only Salmonella isolation between caecal and environmental samples were significantly correlated. Further, one litter sample tested positive for Salmonella before new chicks were introduced. The Campylobacter strains rapidly lost culturability within 2-18 days; in contrast, the Salmonella strains survived from 64-211 days in artificially inoculated water samples. Conclusion: No persistent circulation-mediated Campylobacter contamination was observed. In contrast, circulation of Salmonella in broiler houses was seen, apparently due to the litter excreted from broiler flocks, as well as Salmonella-contaminated water and feed. Significance and Impact of the Study: This paper provides the distribution, genotypic data and circulation dynamics of Campylobacter and Salmonella as recently observed in Japanese chicken broiler farms.

Bibliographic Details

W. Yamazaki; R. Uemura; S. Sekiguchi; J. B. Dong; S. Watanabe; Y. Kirino; H. Mekata; N. Nonaka; J. Norimine; M. Sueyoshi; Y. Goto; Y. Horii; N. Misawa; M. Kurogi; S. Yoshino

Oxford University Press (OUP)

Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology

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