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Loss of insulator activity by paired Su(Hw) chromatin insulators

Science, ISSN: 0036-8075, Vol: 291, Issue: 5503, Page: 495-498
2001
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Article Description

Chromatin insulators are regulatory elements that block the action of transcriptional enhancers when interposed between enhancer and promoter. The Drosophila Suppressor of Hairy wing [Su(Hw)] protein binds the Su(Hw) insulator and prevents enhancer-promoter interaction by a mechanism that is not understood. We show that when two copies of the Su(Hw) insulator element, instead of a single one, are inserted between enhancer and promoter, insulator activity is neutralized and the enhancer-promoter interaction may instead be facilitated. This paradoxical phenomenon could be explained by interactions between protein complexes bound at the insulators.

Bibliographic Details

Ekaterina Muravyova; Anton Golovnin; Elena Gracheva; Aleksander Parshikov; Tatiana Belenkaya; Vincenzo Pirrotta; Pavel Georgiev

American Association for the Advancement of Science (AAAS)

Multidisciplinary

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