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Transcriptional activation by tetracyclines in mammalian cells

Science, ISSN: 0036-8075, Vol: 268, Issue: 5218, Page: 1766-1769
1995
  • 2,137
    Citations
  • 0
    Usage
  • 1,259
    Captures
  • 5
    Mentions
  • 14
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    2,137
    • Citation Indexes
      2,121
    • Patent Family Citations
      15
      • Patent Families
        15
    • Policy Citations
      1
      • Policy Citation
        1
  • Captures
    1,259
  • Mentions
    5
    • References
      3
      • Wikipedia
        3
    • News Mentions
      2
      • News
        2
  • Social Media
    14
    • Shares, Likes & Comments
      14
      • Facebook
        14

Most Recent News

Single-molecule states link transcription factor binding to gene expression

Nature, Published online: 20 November 2024; doi:10.1038/s41586-024-08219-w A study uses single-molecule footprinting to measure protein occupancy at regulatory elements on individual molecules in human cells and describes how different properties of transcription factor binding contribute to gene expression.

Article Description

A transcriptional transactivator was developed that fuses the VP16 activation domain with a mutant Tet repressor from Escherichia coli. This transactivator requires certain tetra cycline (Tc) derivatives for specific DNA binding. Thus, addition of doxycycline to HeLa cells that constitutively synthesized the transactivator and that contained an appropriate stably integrated reporter unit rapidly induced gene expression more than a thousandfold. The specificity of the Tet repressor-operator-effector interaction and the pharmacological characteristics of Tc's make this regulatory system well suited for the control of gene activities in vivo, such as in transgenic animals and possibly in gene therapy.

Bibliographic Details

Manfred Gossen; Sabine Freundlieb; Gabriele Bender; Hermann Bujard; Gerhard Müller; Wolfgang Hillen

American Association for the Advancement of Science (AAAS)

Multidisciplinary

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