PlumX Metrics
Embed PlumX Metrics

Genus-wide assessment of antibiotic resistance in Lactobacillus spp

Applied and Environmental Microbiology, ISSN: 1098-5336, Vol: 85, Issue: 1
2019
  • 255
    Citations
  • 0
    Usage
  • 292
    Captures
  • 1
    Mentions
  • 0
    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Citations
    255
  • Captures
    292
  • Mentions
    1
    • References
      1
      • Wikipedia
        1

Article Description

Lactobacillus species are widely used as probiotics and starter cultures for a variety of foods, supported by a long history of safe usage. Although more than 35 species meet the European Food Safety Authority (EFSA) criteria for qualified presumption of safety status, the safety of Lactobacillus species and their carriage of antibiotic resistance (AR) genes is under continuing ad hoc review. To comprehensively update the identification of AR in the genus Lactobacillus, we determined the antibiotic susceptibility patterns of 182 Lactobacillus type strains and compared these phenotypes to their genotypes based on genome-wide annotations of AR genes. Resistances to trimethoprim, vancomycin, and kanamycin were the most common phenotypes. A combination of homology-based screening and manual annotation identified genes encoding resistance to aminoglycosides (20 sequences), tetracycline (18), erythromycin (6), clindamycin (60), and chloramphenicol (42). In particular, the genes aac(3) and lsa, involved in resistance to aminoglycosides and clindamycin, respectively, were found in Lactobacillus spp. Acquired determinants predicted to code for tetracycline and erythromycin resistance were detected in Lactobacillus ingluviei, Lactobacillus amylophilus, and Lactobacillus amylotrophicus, flanked in the genome by mobile genetic elements with potential for horizontal transfer.

Bibliographic Details

Ilenia Campedelli; Harsh Mathur; Elisa Salvetti; Siobhán Clarke; Mary C. Rea; Sandra Torriani; R. Paul Ross; Colin Hill; Paul W. O’Toole; Johanna Björkroth

American Society for Microbiology

Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Immunology and Microbiology; Environmental Science

Provide Feedback

Have ideas for a new metric? Would you like to see something else here?Let us know