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A host of issues: pseudoreplication in host-microbiota studies

Applied and environmental microbiology, ISSN: 1098-5336, Vol: 90, Issue: 8, Page: e0103324-null
2024
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    Citations
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    Usage
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    Captures
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    Mentions
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    Social Media
Metric Options:   Counts1 Year3 Year

Metrics Details

  • Captures
    1
  • Mentions
    1
    • News Mentions
      1
      • 1

Most Recent News

Researchers at Southeast Missouri State University Release New Data on Applied and Environmental Microbiology (A Host of Issues: Pseudoreplication In Host-microbiota Studies)

2024 AUG 27 (NewsRx) -- By a News Reporter-Staff News Editor at NewsRx Life Science Daily -- Investigators publish new report on Life Sciences -

Review Description

Pseudoreplication compromises the validity of research by treating non-independent samples as independent replicates. This review examines the prevalence of pseudoreplication in host-microbiota studies, highlighting the critical need for rigorous experimental design and appropriate statistical analysis. We systematically reviewed 115 manuscripts on host-microbiota interactions. Our analysis revealed that 22% of the papers contained pseudoreplication, primarily due to co-housed organisms, whereas 52% lacked sufficient methodological details. The remaining 26% adequately addressed pseudoreplication through proper experimental design or statistical analysis. The high incidence of pseudoreplication and insufficient information underscores the importance of methodological reporting and statistical rigor to ensure reproducibility of host-microbiota research.

Bibliographic Details

Hannah McKinnon Reish; Lindsey Dewey; Lucas J. Kirschman; Knut Rudi

American Society for Microbiology

Biochemistry, Genetics and Molecular Biology; Agricultural and Biological Sciences; Immunology and Microbiology; Environmental Science

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