The major outer sheath protein (Msp) of Treponema denticola has a bipartite domain architecture and exists as periplasmic and outer membrane-spanning conformers
Journal of Bacteriology, ISSN: 0021-9193, Vol: 195, Issue: 9, Page: 2060-2071
2013
- 28Citations
- 30Captures
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Metrics Details
- Citations28
- Citation Indexes28
- 28
- CrossRef26
- Captures30
- Readers30
- 30
Article Description
The major outer sheath protein (Msp) is a primary virulence determinant in Treponema denticola, as well as the parental ortholog for the Treponema pallidum repeat (Tpr) family in the syphilis spirochete. The Conserved Domain Database (CDD) server revealed that Msp contains two conserved domains, major outer sheath protein (MOSP) and MOSP, spanning residues 77 to 286 and 332 to 543, respectively, within the N- and C-terminal regions of the protein. Circular dichroism (CD) spectroscopy, Triton X-114 (TX-114) phase partitioning, and liposome incorporation demonstrated that full-length, recombinant Msp (Msp) and a recombinant protein containing MOSP, but not MOSP, form amphiphilic, β-sheet-rich structures with channel-forming activity. Immunofluorescence analysis of intact T. denticola revealed that only MOSP contains surface-exposed epitopes. Data obtained using proteinase K accessibility, TX-114 phase partitioning, and cell fractionation revealed that Msp exists as distinct OM-integrated and periplasmic trimers. Msp folded in Tris buffer contained slightly less β-sheet structure than detergent-folded Msp; both forms, however, partitioned into the TX-114 detergent-enriched phase. CDD analysis of the nine Tpr paralogs predicted to be outer membrane proteins (OMPs) revealed that seven have an Msp-like bipartite structure; phylogenetic analysis revealed that the MOSP and MOSP domains of Msp are most closely related to those of TprK. Based upon our collective results, we propose a model whereby a newly exported, partially folded intermediate can be either processed forOMinsertion by the β-barrel assembly machinery (BAM) or remain periplasmic, ultimately forming a stable, water-soluble trimer. Extrapolated to T. pallidum, our model enables us to explain how individual Tprs can localize to either the periplasmic (e.g., TprK) orOM(e.g., TprC) compartments. © 2013, American Society for Microbiology.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84876178833&origin=inward; http://dx.doi.org/10.1128/jb.00078-13; http://www.ncbi.nlm.nih.gov/pubmed/23457251; https://journals.asm.org/doi/10.1128/JB.00078-13; http://jb.asm.org/cgi/doi/10.1128/JB.00078-13; https://syndication.highwire.org/content/doi/10.1128/JB.00078-13; https://dx.doi.org/10.1128/jb.00078-13; https://journals.asm.org/doi/abs/10.1128/JB.00078-13; http://jb.asm.org/lookup/doi/10.1128/JB.00078-13; https://jb.asm.org/content/195/9/2060; https://jb.asm.org/content/195/9/2060.abstract; https://jb.asm.org/content/195/9/2060.full.pdf; http://jb.asm.org/content/195/9/2060; https://jb.asm.org/content/jb/195/9/2060.full.pdf
American Society for Microbiology
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