Molecular cloning and preliminary genetic analysis of the men gene cluster of Bacillus subtilis.
Journal of bacteriology, ISSN: 0021-9193, Vol: 170, Issue: 6, Page: 2735-2741
1988
- 21Citations
- 5Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Metrics Details
- Citations21
- Citation Indexes21
- CrossRef21
- 14
- Captures5
- Readers5
Article Description
The Bacillus subtilis chromosomal locus that contains the genes encoding the menaquinone biosynthetic enzymes (the men genes) was cloned by using an integrable plasmid vector. The men cluster was reconstituted on three overlapping recombinant plasmids, and a tentative gene order was derived. Evaluations of the direction of transcription and of transcriptional boundaries suggested that the men genes are expressed in the form of at least one polycistronic message. In addition, a spectrum of Men phenotypes resulting from the integration of different internal fragments of the cluster indicate transcriptional complexities, possibly including an internal promoter. The size of cloned DNA fragments required to encompass the transcription unit, as well as the locations of known men mutations within these fragments, suggests that a gene(s) not previously identified with the men system is also located within the cluster. The cloned men sequences make available probes to examine the patterns of transcription from the men locus in response to changing environmental conditions and during the developmental sequence represented by endospore formation.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0024025055&origin=inward; http://dx.doi.org/10.1128/jb.170.6.2735-2741.1988; http://www.ncbi.nlm.nih.gov/pubmed/3131309; https://journals.asm.org/doi/10.1128/jb.170.6.2735-2741.1988; https://dx.doi.org/10.1128/jb.170.6.2735-2741.1988; https://jb.asm.org/content/170/6/2735
American Society for Microbiology
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