Visualization of repair of double-strand breaks in the bacteriophage T7 genome without normal DNA replication
Journal of Bacteriology, ISSN: 0021-9193, Vol: 182, Issue: 2, Page: 327-336
2000
- 3Citations
- 12Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations3
- Citation Indexes3
- CrossRef3
- Captures12
- Readers12
- 12
Article Description
An in vitro system based on extracts of Escherichia coli infected with bacteriophage T7 is able to repair double-strand breaks in a T7 genome with efficiencies of 20% or more. To achieve this high repair efficiency it is necessary that the reaction mixtures contain molecules of donor DNA that bracket the double-strand break. Gaps as long as 1,600 nucleotides are repaired almost as efficiently as simple double-strand breaks. DNA synthesis was measured while repair was taking place. It was found that the amount of DNA synthesis associated with repair of a double-strand break was below the level of detection possible with this system. Furthermore, repair efficiencies were the same with or without normal levels of T7 DNA polymerase. However, the repair required the 5'→3' exonuclease encoded by T7 gene 6. The high efficiency of DNA repair allowed visualization of the repaired product after in vitro repair, thereby assuring that the repair took place in vitro rather than during an in vivo growth step after packaging.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0033988751&origin=inward; http://dx.doi.org/10.1128/jb.182.2.327-336.2000; http://www.ncbi.nlm.nih.gov/pubmed/10629177; http://jb.asm.org/cgi/doi/10.1128/JB.182.2.327-336.2000; https://syndication.highwire.org/content/doi/10.1128/JB.182.2.327-336.2000; https://journals.asm.org/doi/10.1128/JB.182.2.327-336.2000; https://dx.doi.org/10.1128/jb.182.2.327-336.2000
American Society for Microbiology
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