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Localization of the Escherichia coli RNA polymerase β′ subunit residue phosphorylated by bacteriophage T7 kinase Gp0.7

Journal of Bacteriology, ISSN: 0021-9193, Vol: 188, Issue: 10, Page: 3470-3476
2006
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Article Description

During bacteriophage T7 infection, the Escherichia coli RNA polymerase β′ subunit is phosphorylated by the phage-encoded kinase Gp0.7. Here, we used proteolytic degradation and mutational analysis to localize the phosphorylation site to a single amino acid, Thr, in the evolutionarily hypervariable segment of β′. Using a phosphomimetic substitution of Thr, we show that pliosphorylation of β′ leads to increased ρ-dependent transcription termination, which may help to switch from host to viral RNA polymerase transcription during phage development. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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