Bioconversion of C- and C-steroids with parent and mutant strains of Curvularia lunata
Applied Biochemistry and Microbiology, ISSN: 0003-6838, Vol: 46, Issue: 2, Page: 198-205
2010
- 19Citations
- 12Captures
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Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
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Article Description
Regio- and stereospecificity of microbial hydroxylation was studied at the transformation of 3-keto-4-ene steroids of androstane and pregnane series by the filamentous fungus of Curvularia lunata VKM F-644. The products of the transformations were isolated by column chromatography and identified using HPLC, massspectrometry (MS) and proton nuclear magnetic resonance (H NMR) analyses. Androst-4-ene-3,17-dione (AD) and its 1(2)-dehydro- and 9α-hydroxylated (9-OH-AD) derivatives were hydroxylated by the fungus mainly in position 14α, while 6α-, 6β- and 7α-hydroxylated products were revealed in minor amounts. At the transformation of C-steroids (cortexolone and its acetylated derivatives) the presence of 17-acetyl group was shown to facilitate further selectivity of 11β-hydroxylation. Original procedures for protoplasts obtaining, mutagenesis and mutant strain selection have been developed. A stable mutant (M4) of C. lunata with high 11β-hydroxylase activity towards 21-acetate and 17α,21-diacetate of cortexolone was obtained. Yield of 11β-hydroxylated products reached about 90% at the transformation of 17α, 21-diacetate of cortexolone (1 g/l) using mutant strain M4. © 2010 Pleiades Publishing, Ltd.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77952318294&origin=inward; http://dx.doi.org/10.1134/s0003683810020122; http://link.springer.com/10.1134/S0003683810020122; http://link.springer.com/content/pdf/10.1134/S0003683810020122; http://link.springer.com/content/pdf/10.1134/S0003683810020122.pdf; http://link.springer.com/article/10.1134/S0003683810020122/fulltext.html; http://www.springerlink.com/index/pdf/10.1134/S0003683810020122; https://dx.doi.org/10.1134/s0003683810020122; https://link.springer.com/article/10.1134/S0003683810020122; http://www.springerlink.com/index/10.1134/S0003683810020122
Pleiades Publishing Ltd
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