Pharmacological properties of a pore induced by raising intracellular Ca
American Journal of Physiology - Cell Physiology, ISSN: 0363-6143, Vol: 297, Issue: 1, Page: C28-42
2009
- 25Citations
- 37Captures
Metric Options: Counts1 Year3 YearSelecting the 1-year or 3-year option will change the metrics count to percentiles, illustrating how an article or review compares to other articles or reviews within the selected time period in the same journal. Selecting the 1-year option compares the metrics against other articles/reviews that were also published in the same calendar year. Selecting the 3-year option compares the metrics against other articles/reviews that were also published in the same calendar year plus the two years prior.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Example: if you select the 1-year option for an article published in 2019 and a metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019. If you select the 3-year option for the same article published in 2019 and the metric category shows 90%, that means that the article or review is performing better than 90% of the other articles/reviews published in that journal in 2019, 2018 and 2017.
Citation Benchmarking is provided by Scopus and SciVal and is different from the metrics context provided by PlumX Metrics.
Metrics Details
- Citations25
- Citation Indexes25
- CrossRef25
- 22
- Captures37
- Readers37
- 37
Article Description
Recent studies on the P2X receptor in 2BH4 cells and peritoneal macrophages have demonstrated that the raise in intracellular Ca concentration induces a pore opening similar to P2X receptor pore. Herein, we have investigated whether the pore activated by the elevation of intracellular Ca concentration is associated to P2X receptor. Using patch clamp in cell attached, whole cell configuration, and dye uptake, we measured the pore opening in cell types that express the P2X receptor (2BH4 cells and peritoneal macrophages) and in cells that do not express this receptor (HEK-293 and IT45-RI cells). In 2BH4 cells, the stimulation with ionomycin (5-10 μM) increased intracellular free Ca concentration and induced pore formation with conductance of 421 ± 14 pS, half-time (t1?2) for ethidium bromide uptake of 118 ± 17 s, and t1?2 for Lucifer yellow of 122 ± 11 s. P2X receptor antagonists did not block these effects. Stimulation of HEK-293 and IT45-RI cells resulted in pore formation with properties similar to those found for 2BH4 cells. Connexin hemichannel inhibitors (carbenoxolone and heptanol) also did not inhibit the pore-induced effect following the increase in intracellular Ca concentration. However, 5-(N,N-hexamethylene)-amiloride, a P2X receptor pore blocker, inhibited the induced pore. Moreover, intracellular signaling modulators, such as calmodulin, phospholipase C, mitogen-activated protein kinase, and cytoskeleton components were important for the pore formation. Additionally, we confirmed the results obtained for electrophysiology by using the flow cytometry, and we discarded the possibility of cellular death induced by raising intracellular Ca at the doses used by using lactate dehydrogenase release assay. In conclusion, increased concentration in intracellular Ca induces a novel membrane pore pharmacologically different from the P2X associated pore and hemigap-junction pore. Copyright © 2009 the American Physiological Society.
Bibliographic Details
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=67650071224&origin=inward; http://dx.doi.org/10.1152/ajpcell.00476.2008; http://www.ncbi.nlm.nih.gov/pubmed/19321740; https://journals.physiology.org/doi/10.1152/ajpcell.00476.2008; http://ajpcell.physiology.org/cgi/doi/10.1152/ajpcell.00476.2008; http://ajpcell.physiology.org/content/297/1/C28
American Physiological Society
Provide Feedback
Have ideas for a new metric? Would you like to see something else here?Let us know