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Evaluation of hepcidin isoforms in hemodialysis patients by a proteomic approach based on SELDI-TOF MS

Journal of Biomedicine and Biotechnology, ISSN: 1110-7243, Vol: 2010, Page: 329646
2010
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Article Description

The hepatic iron regulator hormone hepcidin consists, in its mature form, of 25 amino acids, but two other isoforms, hepcidin-20 and hepcidin-22, have been reported, whose biological meaning remains poorly understood. We evaluated hepcidin isoforms in sera from 57 control and 54 chronic haemodialysis patients using a quantitative proteomic approach based on SELDI-TOF-MS. Patients had elevated serum levels of both hepcidin-25 and hepcidin-20 as compared to controls (geometric means: 7.52 versus 4.69nM, and 4.06 versus 1.76nM, resp., P <.05 for both). The clearance effects of a single dialysis session by different dialysis techniques and membranes were also investigated, showing an average reduction by 51.3% ± 29.2% for hepcidin-25 and 34.2% ± 28.4% for hepcidin-20 but only minor differences among the different dialysis modalities. Measurement of hepcidin isoforms through MS-based techniques can be a useful tool for better understanding of their biological role in hemodialysis patients and other clinical conditions. © 2010 Natascia Campostrini et al.

Bibliographic Details

Campostrini, Natascia; Castagna, Annalisa; Zaninotto, Federica; Bedogna, Valeria; Tessitore, Nicola; Poli, Albino; Martinelli, Nicola; Lupo, Antonio; Olivieri, Oliviero; Girelli, Domenico

Hindawi Limited

Biochemistry, Genetics and Molecular Biology; Environmental Science

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